HyperScript™ Reverse Transcriptase
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HyperScript™ Reverse Transcriptase is a new enzyme obtained through genetic engineering based on M-MLV Reverse Transcriptase. In comparison, HyperScript™ Reverse Transcriptase reduces RNase H activity and increases thermal stability. HyperScript™ Reverse Transcriptase can withstand higher reaction temperatures and is suitable for reverse transcription of RNA templates with complex secondary structures. In addition, HyperScript™ Reverse Transcriptase enhances the affinity to the template, suitable for the reverse transcription of a small number of templates and low copy genes, and can generate cDNA up to 12.3 kb.
HyperScript™ Reverse Transcriptase is generally used to achieve reverse transcription of RNA into cDNA for subsequent qPCR experiments or other experiments.
| Components | 2000 U | 10,000 U | 40,000 U |
| HyperScript™ Reverse Transcriptase (200 U/μL) | 10 μL | 50 μL | 50 μL x 4 |
| 5X First-Strand Buffer | 500 μL | 500 μL | 500 μL x 2 |
Store the components at -20°C. | |||
Good thermal stability and high reverse transcription efficiency
Suitable for RNA templates with secondary structure
The template has a strong affinity for more full-length cDNA
Highly sensitive to detect total RNA down to 10 pg