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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
HyperScript™ III Reverse Transcriptase is a third-generation reverse transcriptase based on M-MLV Reverse Transcriptase that has been genetically engineered. HyperScript™ III Reverse Transcriptase generates a reduced RNase H activity and improves thermal stability and fidelity compared to wild-type. Therefore, it has characteristics of higher cDNA synthesis yield and longer length, higher reverse transcription efficiency for high-GC content RNA.
This kit is based on HyperScript™ III Reverse Transcriptase, contains all the components required for the synthesis of first-strand cDNA, and provides primers for cDNA synthesis: Random Primers and Oligo(dT)23VN. Random Primers, Oligo(dT)23VN or gene-specific primers can be employed as reverse transcription primers according to your needs, and the synthesized first-strand cDNA products can be used for subsequent PCR amplification, qPCR reactions or other experiments.
Store the components at -20℃ for 2 years.