HyperScript™ III First-Strand cDNA Synthesis SuperMix
HyperScript™ III Reverse Transcriptase is a third-generation reverse transcriptase based on M-MLV Reverse Transcriptase that has been genetically engineered. Compared to wild-type, HyperScript™ III Reverse Transcriptase generates a reduced RNase H activity and improves thermal stability and fidelity. In addition, it has characteristics of higher cDNA synthesis yield and longer length, higher reverse transcription efficiency for high-GC content RNA. HyperScript™ III Reverse Transcriptase has an enhanced affinity for templates and is suitable for reverse transcription of small amounts of template and low-copy genes, you can use PCR to amplify cDNA up to 12.3 kb.
This kit contains all components for the synthesis of first-strand cDNA, and provides two different primers for cDNA synthesis, Random Primers and Oligo (dT)23VN. Random Primers, Oligo(dT)23VN or gene-specific primers can be chosen as reverse transcription primers according to your needs, and the synthesized products can be used for subsequent PCR amplification, qPCR reactions or other experiments.
| Components | 50 rxn (20 μL reaction) | 100 rxn (20 μL reaction) |
| RNase Free ddH2O | 1 mL | 2 X 1 mL |
| 5 X HyperScript™ Ⅲ SuperMix | 200 μL | 400 μL |
| Random Primers (50 μM) | 50 μL | 100 μL |
| Oligo (dT)23VN (50 uM) | 50 μL | 100 μL |
| 5 X HyperScript™ Ⅲ control Mix | 20 μL | 40 μL |
Store the components at -20℃ for 2 years. | ||