HyperTrap DEAE HP Column
HyperChrom DEAE HP Agarose is a weak anion exchange chromatography medium that separates molecules using differences in charge properties and how much they are under specific conditions. It was found that when sodium hydroxide solution was continuously added dropwise to the chromatography medium, measuring the pH of the supernatant found that it could maintain a fairly wide range of pH stability. It is precisely because of this property that HyperChrom DEAE HP Agarose chromatography media exhibits unexpected separation and purification effects. HyperChrom DEAE HP Agarose has excellent scale-up performance: 1) Highly crosslinked agarose base frame with excellent rigidity, so it can achieve high process flow rates and improve process efficiency at low back pressure. 2) Through chemical modification, it has excellent chemical compatibility and is resistant to CIP cleaning such as sodium hydroxide. 3) Fine particle size design to improve resolution.
HyperTrap DEAE HP Column is based on HyperChrom DEAE HP Agarose as the chromatography medium for the isolation and purification of a variety of biomolecules, such as: recombinant proteins, antibodies, nucleic acids, viruses and virus-like particles, polysaccharides, etc.
The main body and inner plug of the pre-installed column tube are made of PP, and the inside and outside are polished, and the sieve plate is made of HDPE material, which has excellent physical properties and chemical resistance, corrosion resistance, anti-aging, long life and good safety. 1) Ready-to-use, convenient and fast. 2) Can be used with syringes, peristaltic pumps or chromatography systems. 3) It can be used in series to increase the sample processing capacity.
1. HyperTrap DEAE HP Column preloaded column parameters
|
Pre-assembled column specification/name |
1 mL |
5 mL |
|
Dimensions (IDxH) |
7.7 x 25 mm |
16 x 25 mm |
|
Withstand pressure |
0.3 MPa |
0.3 MPa |
|
Workflow Speed* |
1 mL/min |
1-3 mL/min |
|
Operating temperature |
4 - 30°C |
4 - 30°C |
|
Storage condition |
Store the components at 4℃ for 5 years. |
|
|
*This flow rate is the recommended common flow rate, the pressure resistance, and the use of different types of chromatography packing in the pre-assembled column will be different, please refer to the product manual of the corresponding chromatographic filler. |
||
2. HyperTrap DEAE HP Column chromatography media parameters
|
Name |
Description |
|
Chromatography media type |
Strong anion exchange |
|
Ligation |
-O-CH2CH2–N⁺ (C2H5)3H |
|
Scaffolding |
Highly cross-linked agarose |
|
Average particle size |
34 μm |
|
Ion load |
0.08-0.13 mmol Cl - /mL chromatography medium |
|
Dynamic load |
>90 mg ovalbumin/mL chromatography medium* |
|
Flow rates are recommended |
90-150 cm/h |
|
Maximum flow rate |
200 cm/h |
|
Withstand pressure |
0.3 MPa |
|
Use temperature |
4-30℃ |
|
pH stability ** |
2-14 |
|
Solvent resistant |
Common aqueous solution, 30% isopropanol***, 75% ethanol***, 1 M NaOH, 1 M acetic acid, 6 M guanidine hydrochloride, 8 M urea |
|
Intolerant to solvents |
Oxidant, anionic detergent |
|
* Dynamic load measurement conditions: column loading height: 10cm, test flow rate 150 cm/h; Test buffer: 0.05 M Tris-HCl solution, pH 8.0; test sample: 6 mg/ml BSA sample, BSA loading volume per volume of media (mL) (mg) when BSA penetration reaches 10%. **After 7 days in the environment of chromatography at 40 °C and pH 2-14, the physical and chemical properties and functions of the chromatography medium did not change significantly. ***30% and 75% are v/v, volume ratio. |
|