Immunoprecipitation Kit (Protein A+G Agarose Gel)

Immunoprecipitation (IP) or co-immunoprecipitation (Co-IP) is the study of proteins or protein-protein interactions. PPIs) are used to separate the target protein from complex samples by using specific antibodies and antibody-binding media (such as Protein A/G agarose gels, etc.), or directly using media conjugated to specific antibodies (such as agarose gels or magnetic beads), which can be used for Western blot detection or mass spectrometry analysis.

Protein A is a cell wall surface protein found in Staphylococcus aureus with a molecular weight of 42 kDa, which specifically binds to the Fc region of mammalian immunoglobulin (Ig) and also to the Fab region of the human VH3 family. Protein G is an immunoglobulin-binding protein expressed by Streptococcal bacteria, which specifically binds to the Fc region of mammalian immunoglobulin (Ig). Protein A can specifically bind to the corresponding antibodies, such as human IgG1, IgG2, IgG4, mouse IgG2a, IgG2b and rabbit IgG, etc., and each Protein A molecule can bind to 5 IgG molecules, while the antibodies that Protein G can bind are human IgG1, IgG2, IgG3, IgG4, mouse IgG1, IgG2a, IgG2b, IgG3, rat IgG1, IgG2a, IgG2b, IgG2c, rabbit, goat polyclonal antibodies, etc., and each Protein G molecule can bind 3 IgG molecules. The binding capacity of common immunoglobulin subclasses and the total binding capacity of different species are shown in the table below (Table 1).

This kit contains Protein A+G agarose gel and other necessary reagents for immunoprecipitation, and with the specific antibodies provided by the user, the immunoprecipitation (IP, also known as pull-down) or co-immunoprecipitation (Co-IP) experiments are simpler, more convenient and more efficient, and the immunoprecipitation products can be used for the detection of the target protein or its protein complexes and other experiments. (See Table 2 for specific product parameters).

Table 1 affinity data for Protein A+G for different sources and subtypes of IgG. ++++ = Strong Binding; ++~+++ = Medium Binding; + = Weak Binding; +/- = Weak or No Binding; - = No Binding.

Table 2 The main related indicators of Protein A+G Agarose Gel.