Ionomycin calcium salt
Ionomycin calcium salt is a calcium ionophore [1].
Ca2+ ionophore acts as a Ca2+ ionophore, allowing Ca2+ to cross cell membranes.
Ionomycin calcium salt is a calcium ionophore. In cultured chicken pectoralis muscle, ionomycin (10-6-10-5 M) selectively increased the incorporation of methionine into two proteins. Ionomycin increased influx of extracellular Ca2+ and increased Ca2+ concentration in the cytoplasm, which then regulated the synthesis of muscle proteins [2]. In rat parotid gland, ionomycin increased 86Rb efflux, 22Na uptake and 3H-protein secretion, which were dependent on increasing cytosolic Ca2+. In parotid cells, ionomycin released the receptor-regulated cellular Ca2+ pool [3]. In human bladder cancer cells HT1376, ionomycin significantly inhibited cell growth in a time- and dose-dependent way and induced apoptotic DNA degradation. Also, ionomycin reduced the ratios of Bcl-2 to Bax mRNA and protein [4].
In athymic nude mice bearing HT1376 tumors, ionomycin significantly inhibited tumor growth and reduced the tumorigenicity [4].
References:
[1]. Liu C, Hermann TE. Characterization of ionomycin as a calcium ionophore. J Biol Chem, 1978, 253(17): 5892-5894.
[2]. Roufa D, Wu FS, Martonosi AN. The effect of Ca2+ ionophores upon the synthesis of proteins in cultured skeletal muscle. Biochim Biophys Acta, 1981, 674(2): 225-237.
[3]. Poggioli J, Leslie BA, McKinney JS, et al. Actions of ionomycin in rat parotid gland. J Pharmacol Exp Ther, 1982, 221(1): 247-253.
[4]. Miyake H, Hara I, Yamanaka K, et al. Calcium ionophore, ionomycin inhibits growth of human bladder cancer cells both in vitro and in vivo with alteration of Bcl-2 and Bax expression levels. J Urol, 1999, 162(3 Pt 1): 916-921.
- 1. Dante Fabros Jr, Wasin Charerntantanakul, et al. "Type I and II interferons, transcription factors and major histocompatibility complexes were enhanced by knocking down the PRRSV-induced transforming growth factor beta in monocytes co-cultured with peripheral blood lymphocytes." Front Immunol. 2024 Mar 6:15:1308330 PMID: 38510257
- 2. Dijin Xu, Weiqian Jiang, et al. "PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection." Nature. 2023 Jul;619(7971):819-827. PMID: 37438530
| Physical Appearance | A crystalline solid |
| Storage | Desiccate at -20°C |
| M.Wt | 747.08 |
| Cas No. | 56092-82-1 |
| Formula | C41H70O9·Ca |
| Solubility | Soluble in DMSO |
| Chemical Name | calcium (4S,6S,8S,10E,12R,14R,16E,18S,19S,20R,21S)-19,21-dihydroxy-22-((2R,2'R,5R,5'S)-5'-((R)-1-hydroxyethyl)-2,5'-dimethyloctahydro-[2,2'-bifuran]-5-yl)-4,6,8,12,14,18,20-heptamethyl-11-oxido-9-oxodocosa-10,16-dienoate |
| SDF | Download SDF |
| Canonical SMILES | O[C@H](C)[C@@]1(C)O[C@H](CC1)[C@]2(C)O[C@@H](C[C@@H]([C@@H](C)[C@H]([C@H](/C=C/C[C@@H](C)C[C@@H](C)/C([O-])=C\C([C@@H](C)C[C@@H](C)C[C@@H](C)CCC([O-])=O)=O)C)O)O)CC2.[Ca+2] |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment:[1] | |
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Cell lines |
Human bladder cancer cell line HT1376 |
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Reaction Conditions |
0 ~ 100 μg/ml ionomycin for 0 ~ 96 h incubation |
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Applications |
The in vitro growth rate of HT1376 cells was suppressed by ionomycin in a dose- and time-dependent manner. After continuous treatment with 10 μg/ml ionomycin, characteristic apoptotic DNA degradation, as well as marked decreases in the ratios of Bcl-2 to Bax mRNA and protein could be observed in HT1376 cells. |
| Animal experiment:[1] | |
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Animal models |
Athymic nude mice bearing HT1376 tumors |
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Dosage form |
50 μg of ionomycin in 100 μl PBS Injected intratumorally three times weekly for 4 weeks |
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Applications |
Intratumoral injection of ionomycin into subcutaneous HT1376 tumors reduced the tumorigenicity in nude mice. Furthermore, these in vivo growth-inhibitory effects of ionomycin were significantly enhanced by pretreatment with cisplatin. |
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Note |
The technical data provided above is for reference only. |
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References: 1. Miyake H, Hara I, Yamanaka K, et al. Calcium ionophore, ionomycin inhibits growth of human bladder cancer cells both in vitro and in vivo with alteration of Bcl-2 and Bax expression levels. The Journal of Urology, 1999, 162(3 Pt 1): 916-921. |
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Quality Control & MSDS
- View current batch:
-
Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
- Datasheet
Chemical structure
