Streptavidin Beads T3
- 1. Zhilin He, Haofei Ji, et al. "Invention of circRNA promoting RNA to specifically promote circRNA production." Nucleic Acids Res. 2024 Aug 9:gkae693 PMID: 39119897
- 2. Yue Zhuo, Shujun Li, et al. "Targeting SNORA38B attenuates tumorigenesis and sensitizes immune checkpoint blockade in non-small cell lung cancer by remodeling the tumor microenvironment via regulation of GAB2/AKT/mTOR signaling pathway." J Immunother Cancer. 2022 May;10(5):e004113. PMID: 35577506
APExBIO Streptavidin Beads T3 are ideal for numerous applications, including purification of proteins and nucleic acids, protein interaction studies, immunoprecipitation, immunoassays, phage display, biopanning, drug screening and cell isolation.
Add the beads to a sample containing biotinylated molecules, e.g. peptides, proteins, antibodies, sugars, lectins, oligonucleotides, DNA/RNA. During a short incubation, the biotinylated molecule will bind to the beads. The molecule-bead complex can be separated by using a magnet. capture, washing, and detection can be optimized for manual or automated use. With indirect capture, mix the biotinylated molecule with the sample to capture the molecule-target complex before adding the beads.
| Name | V (mL) | Concentration | Supplied in | Bead diameter (μm) | Storage | Recommended protein binding amount |
| Streptavidin Beads T3 | 1 or 5*1 mL | 10 mg/mL | PBS, pH 7.4 / 0.1% BSA / 0.02% NaN3 |
3 | Store at 2-8˚C | 10 μg IgG/mg |
| *Note: APExBIO M-280 Streptavidin supplied in phosphate buffered saline (PBS) pH 7.4, with 0.1% bovine serum albumin (BSA) and 0.02% sodium azide as preservatives. | ||||||
Hydrophobic bead surface
Based on tosylactivated beads
Use BSA as blocking proteins
Isoelectric point: pH 5.0
Low charge (–10 mV, at pH 7)
Iron content (ferrites): 12% (17%)
