LDH Cytotoxicity Assay Kit
The LDH Cytotoxicity Assay Kit is a kit that assesses cytotoxicity by measuring the activity of lactate dehydrogenase (LDH) released into the culture medium. LDH is a stable enzyme found in all cells and is released from the cell into the medium when cells are damaged or apoptosis. LDH catalyzes the conversion of lactate to pyruvate, and this catalytic process reduces NAD+ to NADH. The specific substrates provided in this kit can react with the resulting NADH, resulting in a product that is specifically absorbed at 490 nm. This specific absorption is directly proportional to the amount of LDH released into the culture medium Therefore, by measuring the change in absorbance, it is possible to indirectly assess the degree of cell damage or death.
Compared with the traditional 51Cr cytotoxicity test, this kit contains no radioactive substances, which is simpler and safer to use. Moreover, the detection effect of the detection method used in this kit has the same effect as that of 51Cr, and the results are reliable.
Figure 1: LDH release as measured by this kit
| Components |
K2228-200T |
K2228-1000T |
|
Substrate Mix |
1 vial |
5 x 1 vial |
|
Assay Buffer |
1 mL |
5 x 1 mL |
|
Lysis Buffer (10X) |
2.5 mL |
12 mL |
|
Stop Solution |
12 mL |
60 mL |
|
LDH Positive Control |
6 μL |
30 μL |
|
Store the kit at -20°C, stable for 1 year. Substrate Mix should be stored away from light. |
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