Lipase Activity Colorimetric Assay Kit

Lipase is an enzyme that catalyzes fats (lipids) hydrolysis and is a subclass of the esterases. Lipase plays important roles in the digestion, transport and processing of dietary lipids (e.g. oils, triglycerides, fats) in most living organisms. In human digestive system, pancreatic lipase is the key enzyme that converts triglycerides to monoglycerides and free fatty acids. In the damaged pancreas, lipase levels can rise 5 to 10-fold within 24 to 48 hours.

 The Lipase Activity Colorimetric Assay Kit provides a sensitive, easy and fast way for detection of lipase activity in various samples based on colorimetric method. In the assay, lipase in the sample hydrolyzes a triglyceride substrate to produce glycerol, which is quantified enzymatically through monitoring a linked change in the OxiRed probe absorbance (λ = 570 nm). The kit is suited for high throughput screening assay of lipase activity and can detect lipase activity as low as 10 mU/well.