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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Alkaline lysis method separates chromosomal DNA from plasmid DNA based on differences in DNA denaturation and renaturation, making it a widely used method for plasmid DNA preparation. The magnetic bead-based plasmid purification kit uses an improved SDS alkaline lysis method combined with a dual magnetic bead-based plasmid DNA extraction technique. This approach enables stable, efficient, and convenient plasmid DNA extraction. The kit avoids the need for high-speed centrifugation or vacuum filtration, allowing for rapid removal of impurities and specific binding of plasmid DNA.
This kit is suitable for extracting plasmid DNA from 50-200 mL overnight cultures of E. coli and can be used for downstream molecular biology experiments such as DNA sequencing, PCR, cell transfection, in vitro transcription, and restriction enzyme digestion. It supports both manual operation and automated extraction using nucleic acid extraction instruments for high-throughput automation.
Components
K1627-10Tests
Storage
Buffer 1
50mL
Room Temperature
Buffer 2
50 mL
Buffer 3
70 mL
Buffer B1
35 mL
MagBeads
30 mL
4℃
Buffer W1
60 mL
Buffer EB
20 mL
RNase A
1 mL
-20℃
Shipping: Blue Ice Shelf life: 12 months