Micro BCA Protein Assay Kit

BCA stands for Bicinchoninic Acid Assay, which is a protein quantification method based on the biuret principle. Under alkaline conditions, the protein peptide bond reduces Cu²⁺ in copper sulfate to Cu⁺, and then BCA chelates Cu⁺ to form a purple complex that strongly absorbs light at a wavelength of 562 nm. The color of the chelate is related to the protein concentration, and the amount of Cu²⁺ or Cu⁺ obtained is proportional to the amount of protein present in the solution, so the protein concentration to be measured can be obtained by measuring the protein absorbance value.

This kit is designed for protein quantification of low protein concentration samples and can measure the total protein concentration of diluted protein solution (0.5~20 μg/mL). This kit has the characteristics of simple operation, high stability, high sensitivity, and high compatibility, and has good compatibility with a variety of ionic and non-ionic detergents.