Mouse IFN gamma ELISA Kit

IFNγ, also known as IFNG, is a secreted protein that belongs to the type II interferon family. IFNγ is primarily produced by natural killer T cells and natural killer T cells as part of the innate immune response, and CD4 and CD8 cytotoxic T lymphocyte effector T cells also produce IFNγ once antigen-specific immunity is generated. It is a potent activator of macrophages, has antiproliferative effects on transformed cells, and can enhance the antiviral and antitumor effects of type I interferon.

This kit uses bispecific antibody sandwich ELISA method to detect the concentration of mouse IFNγ in the sample, with high sensitivity, strong specificity and good reproducibility. When a standard or sample is added, the mouse IFNγ will bind to the capture antibody, and then the anti-mouse IFNγ with horseradish peroxidase (HRP) is added to form a sandwich immune complex, and finally the chromogenic agent TMB solution is added, and the solid-phase captured HRP catalyzes the oxidation of TMB into a blue substance, and the absorbance value can be quantitatively detected by the microplate reader. By plotting a standard curve and comparing the absorbance values of the sample, the concentration of IFN γ in the sample can be calculated.