Native-PAGE Gel Preparation and Electrophoresis Kit (PI ≤ 7.0)

Native polyacrylamide gel electrophoresis (Native-PAGE) is a polyacrylamide gel electrophoresis on proteins that remain active without adding denaturants such as SDS and phobic ethanol, and is often used for protein identification and purification, such as industrial enzymes. The electrophoresis of natural polyacrylamide gel without SDS can make the biological macromolecules maintain their natural shape and charge during electrophoresis, and their separation is based on the difference of their electrophoretic mobility and the molecular sieve action of the gel, which can obtain higher resolution, especially after electrophoresis separation, it can still maintain the biological activity of biological macromolecules such as proteins and enzymes, which is of great significance for the identification of biological macromolecules.

Various protein molecules have their own isoelectric points due to the different number of basic and acidic amino acids they contain. A low-pH gel system is used for the separation of basic proteins, and a high-pH gel system is used for the separation of acidic proteins. Acidic proteins are usually buffered with a pH of 8.8 in native gel electrophoresis, and the protein will be negatively charged, and the protein will move anode; Electrophoresis for basic proteins is usually carried out in a slightly acidic environment, and the protein is positively charged, so it is necessary to invert the cathode and anode before electrophoresis.

This kit contains all the reagents required for native protein (PI≤ 7.0) gel preparation and electrophoresis, and users only need to bring their own gel preparation utensils and distilled water. This kit can be used to make 30-50 regular-sized pieces of native PAGE gel.