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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Programmed necrosis or necroptosis is a novel type of cell death. When apoptosis conditions are lacking (mainly when the caspase enzyme is inhibited), especially in the presence of caspase inhibitors, the tumor necrosis factor receptor family (TNF-α receptor) as well as the Toll-like receptor family initiate, which then activate two protein kinases, RIP1 and RIP3, ultimately causing cell necrosis. Programmed necrosis is an important cell death mechanism that plays an important role in tumors, neurodegenerative diseases, and inflammation.
The Necroptosis Inducer Kit provides a reagent mixed with recombinant human TNF-α, SM-164 and Z-VAD-FMK (TSZ) in a certain ratio, which can effectively induce programmed necrosis of cells. Recombinant human TNF-α is a pro-inflammatory cytokine with multiple functions; SM-164 is a bivalent mimetic of Smac, which can induce TNF-α-dependent apoptosis; and Z-VAD-FMK is a pan-caspase inhibitor that can penetrate cell membranes. The reagent in this kit is a ready-to-use solution that can be used directly to induce apoptosis. For 6-well plates, using 2 mL of cell culture medium per well, the kit can be used for 100 or 500 samples; and for 96-well plates, using 200 μL of cell culture medium per well, the kit can be used for 1000 or 5000 samples.
Store the reagent at -20°C, stable for 1 year.