Neuregulin/Heregulin-1β (NRG-1β/HRG-1β), human recombinant protein
Neuregulin (NRG) is a signaling protein for ErbB2/ErbB4 receptor heterodimers on the cardiac muscle cells and plays an important role in heart structure and function through inducing cardiomyocyte differentiation. Neuregulin/Heregulin is a family of structurally related polypeptide growth factors derived from alternatively spliced genes (NRG1, NRG2, NRG3 and NRG4). HRG1-β1 contains an Ig domain and an EGF-like domain that is necessary for direct binding to receptor tyrosine kinases ErbB3/ErbB4, which induces ErbB3 and ErbB4 heterodimerization with ErbB2 and leads to tyrosine phosphorylation. HRG1-β1 is involved in up-regulation of expression and function of the autocrine motility-promoting factor (AMF).
Recombinant human Heregulin-β1 (HRG1-β1) is a 7.5 kDa polypeptide consisting of only the EGF domain of heregulin-β1 (65 amino acid residues).
| Determined by the dose-dependent stimulation of the proliferation of human MCF-7 cells. | ||||||
| ED50 | < 0.5 ng/ml. | |||||
| Specific activity | > 2*106 units/mg. | |||||
Quality Control & DataSheet
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Purity: ≥98%, by SDS-PAGE and HPLC.
Endotoxin Level:< 0.1 EU/μg
Related Biological Data
| Gene ID: | 3084 | Accession #: | Q15491 |
| Alternate Names: | NGR beta 1, Heregulin, HRG 1beta, Neuregulin, Neuregulin1 (NRG1-β1), Neu differentiation factor, Pro-neuregulin-1, Acetylcholine receptor-inducing activity, Breast cancer cell differentiation factor p45, Glial growth factor, Heregulin, Neu differentiation factor, Sensory and motor neuron-derived factor. | ||
| Source: | Escherichia Coli. | M.Wt: | 7.86 kDa |
| Appearance: | Lyophilized protein | Storage: | Store at -20°C |
| Formulation: | Sterile Filtered lyophilized (freeze-dried) powder. | ||
| Solubility: | Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. | ||
| Shipping Condition: | Gel pack. | Handling: | Centrifuge the vial prior to opening. |
| Usage: | For Research Use Only! Not to be used in humans. | ||