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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Neutral Red Cell Proliferation Assay Kit is a kit that can quickly detect cell proliferation or toxicity. The uptake of neutral red by cells is determined by the ability of cells to maintain a pH gradient, which is dependent on ATP production. Under physiological pH conditions, neutral red can enter the cell membrane by passive diffusion and accumulate in lysosomes. When the number of cells increases, the intake of neutral red also increases. Conversely, when cells are damaged, their ability to ingest neutral red is diminished. By measuring the amount of neutral red ingested by the cells, the proliferation or toxicity of the cells can be assessed.
In addition, neutral red can also be used as a pH indicator, and its color will change with the pH value. Neutral red appears red in acidic environments, and gradually changes color from red to yellow when the pH rises from 6.8 to 8.0.
Figure 1: Standard curve for this kit
K2262-500T
Neutral Red Solution
10 mL
Neutral Red Lysis Buffer
50 mL
Store the kit at -20°C, stable for 1 year. Neutral Red Solution should be stored away from light.