Nucleic and Cytoplasmic fluid extraction isolation kit
The study of the components of the nucleus and cytoplasm has always been an important direction of cell research, and the components in the cell, especially after the extraction and isolation of the nucleus, can not only realize the research of intracellular protein localization, but also realize the research on the transcriptional regulation mechanism of intranuclear proteins.
This kit provides a simple and convenient method for extracting cytoplasmic and intranuclear proteins from cultured cells and fresh tissue homogenizers, which can be used for subsequent experiments such as Western blot.
Western Blot analysis of cytoplasmic protein β-tubulin and intracellular protein Lamin A of 293T cells isolated by the kit listed above.
| Components | 50 rxns | 100 rxns | 200 rxns |
| Buffer A | 25 mL | 50 mL | 100 mL |
| Buffer B | 0.5 mL | 1 mL | 2 mL |
| Buffer C | 2.5 mL | 5 mL | 10 mL |
| Buffer D | 0.3 mL | 0.6 mL | 1.2 mL |
| Cell wash Buffer | 250 mL | 500 mL | 1 L |
| Protease Inhibitor Cocktail (EDTA-Free, 100X in DMSO)(K1010) | 0.3 mL | 0.6 mL | 1.2 mL |
Buffer A/C and Cell wash Buffer: Store at 4 °C; Others: Store at -20℃. | |||