Oligo (dT) 25 Beads
The mRNA only accounts for 1%-5% of the total RNA in eukaryotic cells, and efficient purification of mRNA is crucial for subsequent experiments. Oligo (dT) 25 Beads are specially designed for the purification of eukaryotic mRNA, which uses monodisperse superparamagnetic beads that covalently couple oligomeric dT sequences on its surface. By base pairing between the oligo DT sequence and the poly(A) tail of the mRNA, high-purity intact mRNA can be quickly separated from eukaryotic total RNA or cells, animal and plant tissues.
The dT sequence combined with mRNA can be directly used as the first chain reaction primer to synthesize cDNA, or mRNA can be eluted from the beads for various downstream molecular experiments, such as RT-PCR, RPA (ribonuclease protection assay), library construction, Northern Blot analysis, next-generation sequencing, etc.
- 1. Pu-Yang Sun, Jie Liu, et al. "Rejuvenation of peripheral immune cells attenuates Alzheimer’s disease-like pathologies and behavioral deficits in a mouse model." Sci Adv. 2024 May 31;10(22):eadl1123 PMID: 38809977
- 2. Mengjun Zhang, Zhuang Gu, et al. "SRRM2 phase separation drives assembly of nuclear speckle subcompartments." Cell Rep. 2024 Mar 26;43(3):113827. PMID: 38381607
- 3. Jiangnan Huang, Linjie Rao, et al. "Effect of crossbreeding and sex on slaughter performance and meat quality in Xingguo gray goose based on multiomics data analysis." Poult Sci. 2023 Aug;102(8):102753. PMID: 37267641
- 4. Pengyu Geng, Jinhui Zhao, et al. "Z-Ligustilide Combined with Cisplatin Reduces PLPP1-Mediated Phospholipid Synthesis to Impair Cisplatin Resistance in Lung Cancer." Int. J. Mol. Sci. 2023, 24, 17046.
| Components | 1 mL | 5*1 mL |
| Oligo (dT) 25 Beads (10 mg/mL) | 1 mL(10 mg) | 5*1 mL(50 mg) |
Store at 4 °C for 12-18 months, please DO NOT freeze | ||