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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
The E. coli that can take up foreign DNA by a process called transformation are known as competent cells. In genetic engineering experiments, competent cells are often used in various DNA transformation experiments.
One Step Competent Cell Preparation Kit is a kit that can quickly prepare a large number of E. coli competent cells. This kit is based on the classical TSS method, which not only retains the convenience of the TSS method, but also improves the transformation efficiency of the competent cells. This kit can transform not only plasmids, but also ligation products, with a transformation efficiency of more than 106 CFU/µg. This kit is very convenient and simple to use, only needing a short time to complete the preparation after strain culture. This kit is suitable for most common E. coli strains such as DH5α, JM109, TG1, HB101 and XL-1. It is also suitable for some E. coli strains used for protein expression or viral plasmid construction, but sometimes it is not as efficient as other common strains when used for transformation of ligation products.
This product should be stored at -20°C, stable for 1 year.