PDGF-AA, murine recombinant protein
The platelet-derived growth factor AA (PDGF-AA) is a member of the PDGF family and is a disulfide-linked homodimer. PDGF family consists of proteins PDGF-A, -B that form three disulfide-linked homodimers (PDGF-AA, -BB and -AB). The PDGFs are stored in platelet α-granules, and are released upon platelet activation. PDGFs signals through receptors PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. However, PDGFR-β interacts with only PDGF-BB and PDGF-AB. PDGF-AA is a potent mitogen for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells and some blood cells.
Recombinant mouse PDGF-AA is a disulfide linked non-glycosylated homodimer comprised of 2 polypeptide chains of 126 amino acids each, with a dimeric molecular weight of 28.9 kDa.
| Determined by the dose-dependent proliferation of Balb/c 3T3 cells and is typically 8 -10 ng/ml. |
Quality Control & DataSheet
- View current batch:
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Purity: ≥97%, by SDS-PAGE and HPLC.
Endotoxin Level:< 0.01 EU/μg
Related Biological Data
| Gene ID: | 18590 | Accession #: | P20033 |
| Alternate Names: | PDGFA, Mouse PDGF-AA, h-PDGF-AA, PDGFAA, PDGF AA, PDGF-AA, rh-PDGF-AA, recombinant mouse PDGF-AA, recombinant PDGF-AA, PDGF. | ||
| Source: | Escherichia Coli. | M.Wt: | 28.9 kDa |
| Appearance: | Lyophilized protein | Storage: | Store at -20°C |
| Formulation: | Lyophilized with no additives. | ||
| Solubility: | Reconstitute in water to a concentration of 0.1-0.5 mg/ml. This solution can then be diluted into other aqueous buffers and stored at +4°C for 1 week and -20°C for future use. | ||
| Shipping Condition: | Gel pack. | Handling: | Centrifuge the vial prior to opening. |
| Usage: | For Research Use Only! Not to be used in humans. | ||