PDGF-BB, murine recombinant protein
The platelet-derived growth factor BB (PDGF-BB) is a member of the PDGF family and is a disulfide-linked homodimer. PDGF family consists of proteins PDGF-A, -B that form three disulfide-linked homodimers (PDGF-AA, -BB and -AB). The PDGFs are stored in platelet α-granules, and are released upon platelet activation. PDGFs signals through receptors PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. However, PDGFR-β interacts with only PDGF-BB and PDGF-AB. PDGF-BB is a potent mitogen for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells and some blood cells.
Recombinant murine PDGF-BB is a homodimeric, non-glycosylated polypeptide of 109 amino acids and having a molecular mass of 24.4 kDa.
| Determined by the dose-dependent proliferation of murine BALB/c 3T3 cells. | ||||||
| ED50 | < 2 ng/ml. | |||||
Quality Control & DataSheet
- View current batch:
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Purity: ≥95%, by SDS-PAGE and HPLC.
Endotoxin Level:< 0.1 ng/μg/span>
Related Biological Data
| Gene ID: | 18591 | Accession #: | P31240 |
| Alternate Names: | PDGFB, Murine PDGF-BB, m-PDGF-BB, PDGFBB, PDGF-BB, PDGF BB, rm-PDGF-BB, recombinant murine PDGF-BB, recombinant PDGF-BB, PDGF. | ||
| Source: | Escherichia Coli. | M.Wt: | 24.4 kDa |
| Appearance: | Lyophilized protein | Storage: | Store at -20°C |
| Formulation: | Lyophilized with no additives. | ||
| Solubility: | Reconstitute in sterile 100mM acetic acid and 0.1% BSA to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. | ||
| Shipping Condition: | Gel pack. | Handling: | Centrifuge the vial prior to opening. |
| Usage: | For Research Use Only! Not to be used in humans. | ||