Hexa His tag peptide
Hexa His is an amino acid sequence consisting of 6 His residues in a row, it is also known as hexa histidine-tag, 6 x His-tag, and by the trademarked name His-tag. Hexa His Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues. The form B is active against certain oligosaccharides. The form S has no measurable activity. Hexa His acts as a metal binding site for a recombinant protein. His-Tag sequence can be placed on the N- or -terminal of a target protein by using vectors from various commercial molecular biology companies. And this Tag is often followed by a cleavage site for a specific protease- for example Leu-Val-Arg-Gly-Ser peptide sequence is recognized and cleaved by the protease known as thrombin.
References:
1. Hengen, P (1995). "Purification of His-Tag fusion proteins from Escherichia coli". Trends in Biochemical Sciences 20 (7): 285-6.
- 1. Apparao B Kummarapurugu, Adam M Hawkridge, et al. "Neutrophil elastase decreases SARS-CoV-2 spike protein binding to human bronchial epithelia by clipping ACE-2 ectodomain from the epithelial surface." J Biol Chem. 2023 Jun;299(6):104820. PMID: 37187291
- 2. Guo M, Chen K, et al. "Bcl-2 mediates coelomocytes apoptosis by suppressing cytochrome c release in Vibrio splendidus challenged Apostichopus japonicus." Dev Comp Immunol. 2019 Oct 26:103533. PMID:31669879
- 3. Guo M, Lv M, et al. "Bax functions as coelomocyte apoptosis regulator in the sea cucumber Apostichopus japonicus." Dev Comp Immunol. 2019 Sep 5:103490. PMID:31494220
- 4. Chang M, Qin Q, et al. "Carboxymethylated polyethylenimine modified magnetic nanoparticles specifically for purification of His-tagged protein." J Sep Sci. 2018 Nov 28. PMID:30488556
- 5. Guo M, Tang X, et al. "Comparative study of the adjuvant potential of four Th0 cytokines of flounder (Paralichthys olivaceus) on an E. tarda subunit vaccine." Dev Comp Immunol. 2018 Sep; 86: 147-155. PMID:29746982
- 6. Bartoschik T, Galinec S, et al. "Near-native,site-specific and purification-free protein labeling for quantitative protein interaction analysis by MicroScale Thermophoresis." Sci Rep. 2018 Mar 21;8 (1): 4977. PMID:29563556
- 7. Guo M, Tang X, et al. "The Immune Adjuvant Effects of Flounder (Paralichthys olivaceus) Interleukin-6 on E.tarda Subunit Vaccine OmpV." Int J Mol Sci. 2017 Jul 5; 18 (7). pii: E1445. PMID:28678171
| Storage | Desiccate at -20°C |
| M.Wt | 840.85 |
| Formula | C36H44N18O7 |
| Synonyms | H-His-His-His-His-His-His-OH |
| Solubility | ≥84.1 mg/mL in DMSO; ≥123.4 mg/mL in EtOH with ultrasonic; ≥67.5 mg/mL in H2O |
| Chemical Name | Hexa His |
| SDF | Download SDF |
| Canonical SMILES | C1=C(NN=C1)CC(C(=O)NC(CC2=CC=NN2)C(=O)NC(CC3=CC=NN3)C(=O)NC(CC4=CC=NN4)C(=O)NC(CC5=CC=NN5)C(=O)NC(CC6=CC=NN6)C(=O)O)N |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Description | Hexa His tag peptide is a 6 x His tag used as a metal binding site for the recombinant protein. | |||||
| Targets | anti-6 x His tag antibody | |||||
| IC50 | ||||||
Quality Control & MSDS
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