Phosphatase Inhibitor Cocktail 1 (100X in DMSO)
Catalog No.
K1012
For serine/threonine protein phosphatases and L-isozymes of alkaline phosphatases.
Featured Products
- 1. Fengwu Zhang, Xiufang Xiong, et al. "RHEB neddylation by the UBE2F-SAG axis enhances mTORC1 activity and aggravates liver tumorigenesis." EMBO J. 2025 Jan 6 PMID: 39762645
- 2. Dang, Derek. "Beyond the Warburg Effect: A Study of Metabolic Alterations in Malignancies of the Posterior Fossa." 2024
- 3. Zhigang Nian, Yingchao Dou, et al. "Interleukin-34-orchestrated tumor-associated macrophage reprogramming is required for tumor immune escape driven by p53 inactivation." Immunity. 2024 Oct 8;57(10):2344-2361.e7. PMID: 39321806
- 4. Aakarsha Rao, Milan S Stosic, et al. "Targeted inhibition of BET proteins in HPV16-positive head and neck squamous cell carcinoma reveals heterogeneous transcriptional responses." Front Oncol. 2024 Sep 5:14:1440836. PMID: 39301555
- 5. Tian Xie, Xi Gu, et al. "Evodiamine ameliorates intervertebral disc degeneration through the Nrf2 and MAPK pathways." Cytotechnology. 2024 Apr;76(2):153-166. PMID: 38495298
- 6. Xiaojing Zhang, et al. "The effects of restraint stress on ceramide metabolism disorders in the rat liver: the role of CerS6 in hepatocyte injury." Lipids Health Dis. 2024 Mar 2;23(1):68. PMID: 38431645
- 7. Qiu-Yue Lin, et al. "Mac-1 deficiency ameliorates pressure overloaded heart failure through inhibiting macrophage polarization and activation." Biochim Biophys Acta Mol Basis Dis. 2024 Mar;1870(3):167048. PMID: 38296117
- 8. Kehan Li, Linan Liu, et al. "Hippo/YAP1 promotes osteoporotic mice bone defect repair via the activating of Wnt signaling pathway." Cell Signal. 2024 Apr:116:111037. PMID: 38184268
- 9. Aakarsha Rao, Zijian Ni, et al. "Targeted inhibition of BET proteins in HPV-16 associated head and neck squamous cell carcinoma reveals heterogeneous transcription response." bioRxiv. 2023 Oct 4:2023.10.02.560587. PMID: 37873389
- 10. Anthony J Domma, Felicia D Goodrum, et al. "Human cytomegalovirus attenuates AKT activity by destabilizing insulin receptor substrate proteins." J Virol. 2023 Oct 31;97(10):e0056323. PMID: 37754763
- 11. Venneti S, Kawakibi A R, et al. "Clinical efficacy of ONC201 in H3K27M-mutant diffuse midline gliomas is driven by disruption of integrated metabolic and epigenetic pathways." Cancer Discovery, 2023: OF1-OF24. PMID: 37584601
- 12. Payal Deepak, Praveen Kumar, et al. "The triglyceride catabolism regulated by a serine/threonine protein phosphatase, Smek1, is required for development and plant infection inMagnaporthe oryzae." Int J Pharm. 2023 Jul 25:642:123160. PMID: 37379892
- 13. Domma A J, Henderson L A, et al. "Human cytomegalovirus attenuates AKT activity by destabilizing insulin receptor substrate proteins." bioRxiv. 2023 Apr 17:2023.04.17.537203. PMID: 37131605
- 14. Mota M, Sweha S R, et al. "Targeting SWI/SNF ATPases in H3. 3K27M diffuse intrinsic pontine gliomas." Proceedings of the National Academy of Sciences, 2023, 120(18): e2221175120. PMID: 37094128
- 15. Lin Y H, Zeng Q, et al. "In vivo screening identifies SPP2, a secreted factor that negatively regulates liver regeneration." Hepatology, 2023: 10.1097. PMID: 37039560
- 16. Xin Ma, Yan Li, Chengyan Chen, et al. "UPF3A is a ubiquitously expressed NMD factor among mouse tissues." bioRxiv. February 18, 2023.
- 17. Jiexin Zhang, Caiwen Ou, et al. "Curcumin attenuates cadmium-induced atherosclerosis by regulating trimethylamine-N-oxide synthesis and macrophage polarization through remodeling the gut microbiota." Ecotoxicol Environ Saf. 2022 Oct 1;244:114057. PMID: 36084504
- 18. Liting Liang, Pan Liu, et al. "L-lactate inhibits lipopolysaccharide-induced inflammation of microglia in the hippocampus." Int J Neurosci. 2022 Jul 26;1-8 PMID: 35880488
- 19. Ni Pan, Zi-Cheng Li, et al. "Antiplatelet and Antithrombotic Effects of Isaridin E Isolated from the Marine-Derived Fungus via Downregulating the PI3K/Akt Signaling Pathway." Mar Drugs. 2021 Dec 24;20(1):23. PMID: 35049878
- 20. Bin Li, Yifan Guo, et al. "Cardiac Overexpression of XIN Prevents Dilated Cardiomyopathy Caused by TNNT2 ΔK210 Mutation." Front Cell Dev Biol. 2021 Jun 17;9:691749. PMID: 34222259
- 21. Xin Zong, Xiao Xiao, et al. "TheN6-methyladenosine RNA-binding protein YTHDF1 modulates the translation ofTRAF6to mediate the intestinal immune response." Nucleic Acids Res. 2021 Jun 4;49(10):5537-5552. PMID: 33999206
- 22. Zhao Huang, Lang Gong, et al. "GS-441524 inhibits African swine fever virus infection in vitro." Antiviral Res. 2021 Jul;191:105081. PMID: 33945807
- 23. Yingming Jiang, Xihu Yu, et al. "ADAMTS19 Suppresses Cell Migration and Invasion by Targeting S100A16 via the NF-κB Pathway in Human Gastric Cancer." Biomolecules. 2021 Apr 12;11(4):561. PMID: 33921267
- 24. Segev Naveh Tassa, Shani Ben Zichri, et al. "A Mechanism for the Inhibition of Tau Neurotoxicity: Studies with Artificial Membranes, Isolated Mitochondria, and Intact Cells." ACS Chem Neurosci. 2021 May 5;12(9):1563-1577. PMID: 33904703
- 25. Chao Jing, Xingchen Li, et al. "The PSMD14 inhibitor Thiolutin as a novel therapeutic approach for esophageal squamous cell carcinoma through facilitating SNAIL degradation." Theranostics. 2021 Apr 3;11(12):5847-5862. PMID: 33897885
- 26. Haibing Zhan, Xuhong Chang, et al. "LncRNA MEG3 mediates nickel oxide nanoparticles-induced pulmonary fibrosis via suppressing TGF-β1 expression and epithelial-mesenchymal transition process." Environ Toxicol. 2021 Jun;36(6):1099-1110. PMID: 33547861
- 27. Yu ZQ, Yi HY, et al. "Ginsenoside Rg1 Suppresses Type 2 PRRSV Infection via NF-κB Signaling Pathway In Vitro, and Provides Partial Protection against HP-PRRSV in Piglet." Viruses. 2019 Nov 10;11(11):1045. PMID: 31717616
- 28. Losier TT, Akuma M, et al. "AMPK Promotes Xenophagy through Priming of Autophagic Kinases upon Detection of Bacterial Outer Membrane Vesicles." Cell Rep. 2019 Feb 19;26(8):2150-2165.e5. PMID: 30784596
Protein phosphorylation is an important covalent post-translational modification that can alter the structural conformation of a protein, which then regulates the function, location and specific binding of the target protein. Many cellular processes are regulated by the reversible phosphorylation of proteins and 30% of the proteins are likely to be phosphorylated at some point during their existence.
Endogenous proteins are produced and degraded in a balanced state, so their cellular levels are stable under stable environmental conditions. Crude cell extracts contain a number of endogenous enzymes, such as phosphatases and proteases, which are capable of degrading and modifying proteins in the extracts. The best way to increase the yield of intact proteins is to add inhibitors of those enzymes known to be present.
Phosphatase Inhibitor Cocktail 1 inhibits L-isozymes of alkaline phosphatases and serine/threonine protein phosphatases such as protein phosphatases 1 and 2A (PP1 and PP2A). This phosphatase inhibitor cocktail has been optimized and tested on cell extracts from various animal tissues.
This phosphatase inhibitor cocktail contains individual components, including Cantharidin, Bromotetramisole and Microcystin LR. This phosphatase inhibitor cocktail is supplied as a ready-to-use solution in DMSO.
Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts) before assaying.
Applications: WB, Co-IP, pull-down, IF, IHC, kinase assay and etc.
| Catalog No. | Product Name | Summary | Targets | CAS Number | Smiles |
| N1686 | Cantharidin | Inhibitor of protein phosphatases 1/2A | Natural Products | 56-25-7 | O=C([C@@]1(C([H])([H])[H])[C@]2(C([H])([H])[H])[C@@]3([H])O[C@]1([H])C([H])([H])C3([H])[H])OC2=O |
| B4750 | (-)-p-Bromotetramisole Oxalate | ALP inhibitor, potent and non-specific | Others|Others | 62284-79-1 | BrC(C=C1)=CC=C1[C@H]2N=C3SCCN3C2.OC(C(O)=O)=O |
| B3698 | Microcystin-LR | Inhibitor of protein phosphatase PP1/PP2A,potent and selective | Chromatin/Epigenetics|Protein Ser/Thr Phosphatases | 101043-37-2 | CC1C(NC(=O)C(NC(=O)C(C(NC(=O)C(NC(=O)C(NC(=O)C(=C)N(C(=O)CCC(NC1=O)C(=O)O)C)C)CC(C)C)C(=O)O)C)CCCN=C(N)N)C=CC(=CC(C)C(CC2=CC=CC=C2)OC)C |
| Stored at -20°C, and stable for at least 12 months. When kept at 2-8°C, the product remains stable for 2 months. | |||||
Stored at -20°C, and stable for at least 12 months. When kept at 2-8°C, the product remains stable for 2 months.
