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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
PMA qPCR live bacteria detection kit provides an effective method for detecting bacterial viability. The kit includes PMA dye and a SYBR Green-based qPCR mix. PMA is a high-affinity DNA-binding dye that primarily binds to double-stranded DNA and emits bright fluorescence upon binding. Since PMA cannot penetrate cell membranes, it only modifies the DNA of dead cells with damaged membranes. After exposure to blue light (~464 nm), the photosensitive azide groups on PMA are converted into highly reactive nitrene radicals, forming stable covalent nitrogen-carbon bonds, which result in permanent modification of the DNA, making it insoluble and lost during subsequent genomic DNA extraction. Unbound PMA decomposes into non-crosslinking hydroxylamine compounds under strong light and no longer binds to DNA. Thus, leveraging PMA's properties, the PMA-qPCR method combines PMA with qPCR technology for a novel detection approach, used for live bacterial screening. This method has been validated in various bacteria, yeasts, fungi, viruses, and parasites.
Components
K1543-20tests
K1543-200tests
Storage
PMA Dye, 20 mM in H2O
10 μL
100 μL
-20°C, protect from light
Universal SYBR Green qPCR Super mix
0.2 mL
2 mL
Shipping: Blue Ice Shelf life: 12 months