Polybrene (10 mg/mL)

Polybrene, also known as Hexadimethrine Bromide, is a cationic polymer widely used in retrovirus-mediated or lentivirus-mediated gene transfection. The mechanism of action may be to neutralize electrostatic repulsion between cell surface acid and viral particles. In addition, Polybrene can also be used in DNA transfection to enhance the transfection efficiency of liposomes. Meanwhile, Polybrene is an anti-heparin agent and can be used to produce non-specific agglutinated red blood cells. At the same time, Polybrene can be used in protein sequencing to enhance peptide degradation. The addition of Polybrene to the PVDF membrane can also improve the affinity of the membrane.

This product is supplied as a 10 mg/mL stock solution dissolved in 0.9% NaCl and sterilized by a 0.22 μM filter. Polybrene may be toxic to some cells, and long-term treatment (> 12 h) may also be toxic to some cells. Therefore, it is recommended to perform a toxicity test for the first time using.