Polyethylenimine Linear(PEI), MW40000
Linearized polyethyleneimine PEI (molecular weight 40,000) is a highly charged cationic polymer that readily binds to DNA or other negatively charged biomacromolecules, making it a common and effective cell transfection reagent. In principle, PEI condenses DNA plasmid into positively charged complexes. The complexes can adhere to negatively charged cell surface residues, and then enters the cell through endocytosis.
As a transient transfection reagent, PEI has the advantages of high efficiency, low cost and relative stability, etc., which has been validated for a wide range of common cell lines including HEK-293, HEK293T, CHO-K1, HepG2 and Hela cell transfection. In HEK293 and CHO cell expression systems, PEI provides excellent transfection results at different sizes (from 96-well plates to 100 L bioreactors).
- 1. Jing Li, Siyu Li, et al. "H3K18 lactylation-mediated nucleotide-binding oligomerization domain-2 (NOD2) expression promotes bilirubin-induced pyroptosis of astrocytes." Research Square. 12 Sep, 2024
- 2. Roach, Arantxa, et al. "Kidney-Targeted mRNA Nanoparticles: Exploration of the mRNA Loading Capacity of a Polymeric Mesoscale Platform Employing Various Classes of Excipients." (2024). Biochemistry and Molecular Biology. 2.
- 3. Yu Huang, Lu Li, et al. "WHIM Syndrome: Report of a Novel Familial CXCR4V340fs Gain-of-function Mutation with a Milder Phenotype." Research Square. 13 Nov, 2023.
| Components | 4 mL (2.5 mg/mL) | 8 mL (2.5 mg/mL) |
| PEI-2.5 mg/mL | 4 mL | 8 mL |
| 2X Transfection Buffer | 3 mL | 6 mL |
For long-term storage, store at -20°C. For frequent use, it can be placed at 4°C to avoid repeated freeze-thaw cycles. | ||