PreScission Protease (PSP)
- 1. Rachana Tomar, Pratibha P Ghodke, et al. "DNA Replication across α-l-(3′-2′)-Threofuranosyl Nucleotides Mediated by Human DNA Polymerase η." Biochemistry. 2024 Oct 1;63(19):2425-2439. PMID: 39259676
- 2. Megan Jr Yeo, Olivia Zhang, et al. "Asymmetric Engagement of Dimeric CRL3KBTBD4 by the Molecular Glue UM171 Licenses Degradation of HDAC1/2 Complexes." bioRxiv. 2024 May 14:2024.05.14.593897. PMID: 38798619
- 3. Michael P. Stone, et al. "Replication Bypass of the N-(2-Deoxy-d-erythro-pentofuranosyl)-urea DNA Lesion by Human DNA Polymerase η." Biochemistry. 2024 Mar 19;63(6):754-766. PMID: 38413007
- 4. Robyn Richie-Jannetta, Pradeep Pallan, et al. "The peroxidation-derived DNA adduct, 6-oxo-M1dG, is a strong block to replication by human DNA polymerase η." J Biol Chem. 2023 Jul 17;105067. PMID: 37468099
- 5. Xiaoying Wei, Zhishuo Wang, et al. "Human TDP1, APE1 and TREX1 repair 3′-DNA–peptide/protein cross-links arising from abasic sites in vitro." Nucleic Acids Res. 2022 Apr 22;50(7):3638-3657. PMID: 35349719
- 6. Jennifer Carlson. "Regulation of epigenetics and development by the xenobiotic/oxidative stress response factor Keap1 in Drosophila." University of Minnesota. July 2022.
PreScission Protease is a recombinant protease that combines human rhinovirus type 14 3C protease and GST. This protease can specifically recognize the short peptide Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro at low temperature (4°C) and digest between Gln and Gly amino acid residues. Its substrate recognition and cleavage depend not only on the primary structure of the recombinant protease but also on its secondary structure and tertiary structure. It specifically isolates the heterologous proteins expressed by the vectors such as pGEX-6p series from its fused GST label to obtain heterologous target proteins with higher purity.
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Components |
K1101-100U |
K1101-200U |
K1101-500U |
Storage |
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PreScission Protease |
100 U |
200 U |
500 U |
-80°C |
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10×Cleavage Buffer |
1 mL |
2x1 mL |
3x1 mL |
-80°C |
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Shipping: Dry Ice Shelf life: 2 years |
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Note: It is recommended to aliquot upon first use to avoid repeated freeze-thaw cycles: Store aliquots at -20°C for a validity period of 6 months.
Sterile colorless liquid
used at low temperatures (4°C)
specifically recognize the short peptide Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro at low temperature (4°C) and digest between gln and gly amino acid residues.