Protease Inhibitor Cocktail for bacterial cell extracts (100 X in DMSO)
Endogenous proteins are produced and degraded in a balanced state, so their cellular levels are stable under stable environmental conditions. Crude cell extracts contain several endogenous enzymes, such as phosphatases and proteases, which are capable of degrading proteins in the extracts. Protein production is greatly halted and degradation is increased when proteins are extracted from cells and tissues in vitro. The best way to increase the yield of intact proteins is to add inhibitors of those enzymes known to be present.
Protease Inhibitor Cocktail for bacterial cell extracts (100 X in DMSO) is used with bacterium extracts to increase protein stability. The cocktail functions to inhibit proteases that would degrade either non-phosphorylated or phosphorylated protein substrates.
This protease inhibitor cocktail contains individual components, including AEBSF, Bestatin, E-64 and Pepstatin A with a broad specificity for cysteine, aminopeptidase, serine, aspartic, and metalloproteases. This cocktail has been optimized and tested for bacterium cell use. This protease inhibitor cocktail is supplied as a ready-to-use solution in DMSO.
Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as bacterial cell extracts) before assaying.
Applications: WB, Co-IP, pull-down, IF, IHC, kinase assay and etc.
| Tube | Catalog No. | Product Name | Summary | Targets | CAS Number | Smiles |
| A (100X in DMSO) | ||||||
| A2573 | AEBSF.HCl | Serine protease inhibitor | Proteases|Serine Protease | 30827-99-7 | C1=CC(=CC=C1CCN)S(=O)(=O)F.Cl | |
| A2575 | Bestatin | Aminopeptidase inhibitor | Proteases|Aminopeptidase | 58970-76-6 | CC(C)CC(C(=O)O)NC(=O)C(C(CC1=CC=CC=C1)N)O | |
| A2576 | E-64 | Cysteine protease inhibitor,irreversible | Proteases|Cathepsin | 66701-25-5 | CC(C)CC(C(=O)NCCCCN=C(N)N)NC(=O)C1C(O1)C(=O)O | |
| A2571 | Pepstatin A | Aspartic proteinases inhibitor | Proteases|Other Proteases | 26305-03-3 | CC(C)CC(C(CC(=O)O)O)NC(=O)C(C)NC(=O)CC(C(CC(C)C)NC(=O)C(C(C)C)NC(=O)C(C(C)C)NC(=O)CC(C)C)O | |
| B (100X in ddH2O) | ||||||
| EDTA, disodium salt, dihydrate | Metalloprotease inhibitor | protease | 6381-92-6 | |||
| Stored at -20°C, and stable for at least 12 months. | ||||||