Flag-tag Protein IP Assay Kit (Magnetic Beads)
The Flag tag is a polypeptide consisting of 8 amino acid residues (DYKDDDDK), usually in the form of Flag or 3X Flag. It can be linked to the 5' or 3' end of the gene of interest through genetic recombination technology to form a target protein with a Flag tag. Flag tags are widely used in the study of protein expression, purification, identification, interaction and function because they do not interact with the target protein and do not affect the function of the target protein, the N-terminal Flag tag can be excised by enterokinase to obtain an untagged target protein, and can be detected and purified by Flag antibody, Anti-Flag magnetic beads or Anti-Flag affinity gel.
Anti-DYKDDDDK (Flag) Magnetic Beads is a biological experiment tool designed for life science research. It is composed of a high-quality Flag monoclonal antibody covalently conjugated to nanoscale amino magnetic beads, which is immobilized on the surface of the beads to specifically bind to the protein containing the Flag tag, and then through magnetic separation technology, the target protein can be rapidly purified and detected.
This kit contains high-quality Anti-Flag magnetic beads and optimized and validated necessary reagents for immunoprecipitation, making immunoprecipitation (IP, also known as pull-down) or co-immunoprecipitation (Co-IP) experiments easier and more efficient, and widely used for immunoprecipitation, co-immunoprecipitation, or purification of Flag-tagged fusion proteins or their protein complexes. It can be applied to a variety of sample types, such as cell lysates, cell secretion supernatants, serum, animal ascites, and other immune antigens.
This product can be used for immunoprecipitation (IP) and co-immunoprecipitation (Co-IP). Available in 10 tests and 50 tests (e.g. 20 μL per bead usage).
Product parameters
|
Type of antibody |
Anti-Flag Tag monoclonal antibody |
|
Bead particle size |
1 μm |
|
Magnetic bead concentration |
10 mg/mL |
|
Ability to bind |
≥ 0.6 mg DYKDDDDK-tagged fusion protein/mL of beads. |
|
Scope of application |
IP、Co-IP |
|
Components |
10 tests |
50 tests |
Storage |
|
Anti-DYKDDDDK (Flag) Magnetic Beads |
200 μL |
1 mL |
4°C |
|
Cell Lysis Buffer |
5 mL |
25 mL |
4°C |
|
10X TBS |
5 mL |
30 mL |
4°C |
|
Protease Inhibitor Cocktail (100X) |
50 μL |
250 μL |
-20°C |
|
5X Protein Loading Buffer (Reducing) |
200 μL |
1 mL |
-20°C |
|
Acid Elution Buffer |
1 mL |
5 mL |
4°C |
|
Neutralization Buffer |
100 μL |
500 μL |
4°C |
|
3X Flag Peptide（25X） |
80 μL |
0.4 mL |
-20°C |
|
Shipping: Blue ice |
Shelf life: 12 months |
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