Biotin Labeling Kit for Avi-tag Protein (BirA)

The Avi tag is a short peptide tag composed of 15 amino acids, and BirA is able to link biotin to the lysine residue of the Avi tag in the presence of ATP and biotin to achieve biotin labeling of the protein of interest. This method has several advantages, including small Avi tag and low impact on fusion proteins, high biotin labeling efficiency and good reproducibility, and high affinity of the labeled protein to Streptavidin.

After AVI tag protein biotin labeling, it can be applied to a variety of biological detection methods, such as immunofluorescence, in situ hybridization, or flow cytometry, for visualization and quantification. In addition, the binding system of biotin and streptavidin has the advantages of high affinity, high sensitivity, strong specificity and good stability, and is widely used in a variety of detection systems.

The Biotin Labeling Kit for Avi-tag Protein (BirA) is a tool for biotin labeling of proteins or peptides fused with Avi tags using biotin ligase (BirA). This kit enables efficient and fast biotin labeling, which can be used to detect and purify target proteins using Streptavidin or Avidin.