His-tag Protein Purification Kit (Reductant and Chelator Resistant)

His-tagged protein purification kit is a tool for the extraction of His-tagged recombinant proteins from cells or cell lysates. These kits typically contain affinity chromatography media, such as Ni-Agarose or Ni-NTA resins, that specifically bind to histidine residues on the His-tag. When using the His-tagged protein purification kit, the protein sample is passed through an affinity chromatography medium, the target protein is adsorbed, while the other proteins are not bound, and the target protein can be eluted off by changing buffer conditions after the impurities are removed by washing.

This product is a His-tagged protein purification kit for reducing agent and chelating dosage form, which can well tolerate reducing agents such as DTT and chelating agents such as EDTA, such as reducing agents such as 20 mM DTT and chelating agents such as 20 mM EDTA, providing the possibility for the purification of protein samples that require the addition of reducing agent or chelating agent. However, this product is not compatible with 8 M urea and 6 M guanidine hydrochloride, for the purification of inclusion body proteins, in the case of 8 M urea or 6 M guanidine hydrochloride solubilized protein, urea and guanidine hydrochloride need to be removed by dialysis before this product can be used for purification of His-tagged proteins.

This product is sufficient for 10 purifications of His-tagged recombinant proteins, with a wet weight of approximately 1 gram per bacterial pellet. The maximum purified amount of protein is 10-15 mg, and the specific maximum purification amount is related to the molecular weight of the protein. For a 50 kD His-tagged recombinant protein, the maximum amount of protein purified is 3-10 mg per transaction. Without the use of affinity chromatography column empty column tubes, this kit is sufficient for 500 purifications of small amounts of His-tagged proteins.