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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Protein Transport Inhibitors (500X) is a pre-mixture cocktail of brefeldin A and monensin. Brefeldin A and monensin are protein transport inhibitors that allow secreted proteins to accumulate in the endoplasmic reticulum and Golgi, allowing the resulting cytokines to remain in the cell. It can be used as a negative control for the Cell Stimulation Cocktail (Protein Transport Inhibitors Plus, 500X).
This reagent is supplied as a stock solution dissolved in ethanol and can be used directly. The concentration of brefeldin A is 5.3 mM (1.5 mg/mL), and monensin is 1 mM (0.7 mg/mL).
Components
K2717-100 μL
Protein Transport Inhibitors (500X)
100 μL
Store the reagent at -20°C away from light, stable for 1 year.