Recombinant Human G-CSF
Granulocyte colony stimulating factor (G-CSF) is a pleiotropic cytokine. It is mainly produced by monocytes and macrophages upon activation by endotoxin, TNF-α and IFN-γ. Besides, many other cell types can secreted this protein after LPS, IL-1 or TNF-α activation, which are fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells. Various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. G-CSF is cytokine that acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. In addition it may function in some adhesion or recognition events at the cell surface. In humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino acid (a.a.) precursor proteins, have been isolated. Both proteins have a 30 a.a. signal peptide and have identical amino acid sequences except for a three a.a. insertion (deletion) at the 35th a.a. residue from the N-terminus of the mature protein. Human G-CSF is 73 % identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.
Reference:
1. Scarffe JHandKamthan A. 1990. Cancer Surv, 9: 115-30.
2. Fattorini L, Xiao Y, Li B, et al. 1994. J Med Microbiol, 40: 129-33.
3. Tsavaris N, Kosmas C, Gouveris P, et al. 2004. Med Sci Monit, 10: PI24-8.
4. Shochat E, Rom-Kedar V, Segel LA. 2007. Bull Math Biol, 69: 2299-338.
5. Cornish AL, Campbell IK, McKenzie BS, et al. 2009. Nat Rev Rheumatol, 5: 554-9.
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Gene ID |
1440 |
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Accession # |
P09919 |
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Alternate Names |
Granulocyte Colony Stimulating Factor, CSF-3, MGI-1G, GM-CSF beta, Pluripoietin |
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Source |
Escherichia coli. |
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M.Wt |
Approximately 18.7 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids. |
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AA Sequence |
TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP |
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Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70°C as supplied. - 1 month, 2 to 8°C under sterile conditions after reconstitution. - 3 months, -20 to -70°C under sterile conditions after reconstitution. |
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Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in 10mM sodium acetate buffer, containing 5% trehalose, pH 4.0. |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
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Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine NFS-60 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0×107 IU/mg. |
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Shipping Condition |
Gel pack. |
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Handling |
Centrifuge the vial prior to opening. |
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Usage |
For Research Use Only! Not to be used in humans. |
Quality Control & MSDS
- View current batch:
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Purity >98% by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 1 EU/μg of rHuG-CSF as determined by LAL method.