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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Interon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction [1, 2]. IFN-alpha has both anti-viral and immunomodulatory activities on target cells. Type I Interferons (IFNs) are well-known cytokines that exert antiviral activity, antitumor activity, and immunomodulatory effects. Interferon tau (IFNT), a type I IFN similar to alpha IFNs (IFNA), is the pregnancy recognition signal produced by the ruminant conceptus. Among the IFN-α genes, a total of 28 different sequence variants have been described. The three principal subtypes of IFNα-2 are designated α-2a, α-2b, and α-2c. IFNα-2b is being the predominant allele while IFNα-2a is less predominant and IFNα-2c only a minor allelic variant [2, 3].
Reference
[1]. Wang. et al. (2004) J Neuroimmunol. 156(1-2): 107-12.
[2]. Groopman JE, et al. (1984) Ann Intern Med. 100(5): 671-6.
[3]. Krueger JM, et al. (1987) Int J Immunopharmacol. 9(1): 23-30.
Accession #
V00549
Alternate Names
Human IFNA2; IFNA2a; IFNalpha 2; IFN-alpha 2; IFN-alphaA; INFA2; interferon alpha A
Source
Human embryonic kidney cell, HEK293-derived human IFN-alpha A protein
Protein sequence
Cys24-Glu188
M.Wt
19.2 kDa
Appearance
Solution protein.
Stability & Storage
Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage. 12 months from date of receipt, -20 to -70 °C as supplied.
Concentration
0. 2 mg/mL
Formulation
Dissolved in sterile PBS buffer.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. This solution can be diluted into other aqueous buffers.
Biological Activity
Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus (VSV). The EC50 for interferon in this assay is 0.5-3 pg/mL
Shipping Condition
Shipping with dry ice.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans.