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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
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Interleukin-28A (IL-28A), IL-28B, and IL-29, also named interferon-lambda 2 (IFN-lambda 2), IFN-lambda 3, and IFN-lambda 1, respectively, are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11-13% aa sequence identity) and type I IFN family (15-19% aa sequence identity) [1-3]. The genes encoding these three cytokines are localized to chromosome 19 and each is composed of multiple exons. The exon organization of these genes is also found in the IL-10 family genes but is distinct from the type I IFNs, which are encoded within a single exon. The expression of IL-28A, B, and IL-29 is induced by virus infection or double-stranded RNA. All three cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The three proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL-10 R beta) and a novel IL-28 receptor alpha (IL-28 R alpha, also known as IFN-lambda R1). Ligand binding to the receptor complex induces Jak kinase activation and STAT1 and STAT2 tyrosine phosphorylation. The phosphorylated STAT1 and STAT2 complex with IFN-regulatory factor 9 (IRF-9) to form the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex that is translocated to the nucleus. ISGF -3 binds to the IFN-stimulated response element (ISRE) present in the regulatory regions of the target genes. Human IL-28A cDNA encodes a 200 amino acid (aa) residue precursor protein with a putative 25 aa signal peptide. It shares 94% and 67% aa sequence identity with human IL-28B and human IL-29, respectively.
Reference
[1]. Durbin, R.K. et al. (2013) Immunol. Rev. 255:25.
[2]. Li, Q. et al. (2013) Front. Biosci. (Landmark Ed.) 18:909.
[3]. Lopusna, K. et al. (2013) Acta Virol. 57:171.
Accession #
Q8IZJ0
Alternate Names
interleukin-28A; IFN-lambda 2; IL28A; IL-28A; interferon, lambda 2
Source
Human embryonic kidney cell, HEK293-derived human IL-28A/IFN-lambda 2 protein
Protein sequence
Val26-Val200
M.Wt
19.8 kDa
Appearance
Solution protein.
Stability & Storage
Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage. 12 months from date of receipt, -20 to -70 °C as supplied.
Concentration
0. 2 mg/mL
Formulation
Dissolved in sterile PBS buffer.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. This solution can be diluted into other aqueous buffers.
Biological Activity
Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. The EC50 for this effect is 5-20 ng/mL.
Shipping Condition
Shipping with dry ice.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans.