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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Interleukin-3 (IL-3) is a potent growth-promoting cytokine that belongs to the IL-3 family. IL3/IL-3 also belongs to the group of interleukins. Interleukins are produced by a wide variety of body cells. The function of the immune system depends in a large part on interleukins, and rare deficiencies of a number of them have been described, all featuring autoimmune diseases or immune deficiency. The majority of interleukins are synthesized by helper CD4+ T lymphocytes, as well as through monocytes, macrophages, and endothelial cells. They promote the development and differentiation of T, B, and hematopoietic cells. IL3/IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation, and apoptosis. IL3/IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
Reference
[1]. Meyer CG, et al. (2011) Hum Mol Genet. 20(6):1173-81.
[2]. Zambrano A, et al. (2010) Curr Alzheimer Res. 7(7):615-24.
[3]. Dentelli P, et al. (2011) Oncogene. 30(50):4930-40.
Accession #
AAC08706
Alternate Names
Human IL3; IL3; IL-3; recombinant IL3; interleukin-3; Mast cell growth factor
Source
Human embryonic kidney cell, HEK293-derived human IL3 protein
Protein sequence
Ala20-Phe152
M.Wt
15.1 kDa
Appearance
Solution protein.
Stability & Storage
Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage. 12 months from date of receipt, -20 to -70 °C as supplied.
Concentration
0. 2 mg/mL
Formulation
Dissolved in sterile PBS buffer.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. This solution can be diluted into other aqueous buffers.
Biological Activity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The EC50 for this effect is 0.01-0.08 ng/mL.
Shipping Condition
Shipping with dry ice.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans.