Recombinant Human MHC Class I Polypeptide-Related Sequence A, His
MIC-A (MHC class I chain-related gene A) is a single-pass type I member protein. It is expressed on the cell surface in gastric epithelium, endothelial cells and fibroblasts and in the cytoplasm in keratinocytes and monocytes. Additionally, MIC-A can be induced by bacterial and viral infections. It shares 85 % amino acid identity with MIC-B and they are distantly related to the MHC class I proteins. Because they possess three extracellular Ig-like domains, but unlike classical MHC class I molecules. They do not form a heterodimer with beta2 microglobulin, but bind as a monomer to a KLRK1/NKG2D that is an activating receptor expressed on NK cells, NKT cells, γδ T cells, and CD8+ αβ T cells. Recognition of MICA by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MIC-A recognition plays an important role in tumor surveillance, viral infections, and autoimmune diseases.
Reference
1. Yao Z, Volgger A, Helmberg W, et al. 1999. Eur J Immunogenet, 26: 225-32
2. Li P, Willie ST, Bauer S, et al. 1999. Immunity, 10: 577-84
3. Petersdorf EW, Shuler KB, Longton GM, et al. 1999. Immunogenetics, 49: 605-12
4. Komatsu-Wakui M, Tokunaga K, Ishikawa Y, et al. 1999. Immunogenetics, 49: 620-8
5. Gambelunghe G, Ghaderi M, Cosentino A, et al. 2000. Diabetologia, 43: 507-14.
| Source | Escherichia coli. |
| M.Wt | Approximately 36.9 kDa, a single non-glycosylated polypeptide chain containing 320 amino acids. |
| AA Sequence | MSYYHHHHHH DYDIPTTENL YFQGAMDPEF EPHSLRYNLT VLSWDGSVQS GFLAEVHLDG QPFLRYDRQK CRAKPQGQWA EDVLGNKTWD RETRDLTGNG KDLRMTLAHI KDQKEGLHSL QEIRVCEIHE DNSTRSSQHF YYDGELFLSQ NLETEEWTVP QSSRAQTLAM NVRNFLKEDA MKTKTHYHAM HADCLQELRR YLESGVVLRR TVPPMVNVTR S |
| Appearance | Sterile Colorless liquid. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Refer to lot specific COA for the Use by Date when stored at ≤ -20 °C as supplied. |
| Formulation | Supplied as a 0.2 μm filtered concentrated sterile solution in PBS, pH 7.4, and 8 M Urea. |
| Biological Activity | Fully biologically active when compared to standard. The specific activity is determined by binding MICA antibody in ELISA. |
| Shipping Condition | Blue ice |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
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Purity > 95 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 1 EU/µg of rHuMIC-A, His as determined by LAL method.