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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Activins and Inhibins are TGF-β superfamily cytokines that are involved in tissue morphogenesis and repair, fibrosis, inflammation, neural development, hematopoiesis, reproductive system function, and carcinogenesis. Activin A protects the heart from hypoxic stress and promotes the differentiation of embryonic stem cells into cardiomyocytes. Activins are homodimers or heterodimers of various β subunits (βA, βB, βC, and βE), while Inhibins are heterodimers of a unique α subunit and one of the β subunits. Activin A is a homodimer of two βA chains. Human βA shares 100% amino acid sequence identity with mouse, rat, bovine, porcine, and feline βA. Activin A binds to Activin RIIA which then associates with Activin RIB/ALK-4. Activin A bioactivity is regulated by cell-associated molecules (BAMBI, Betaglycan, and Cripto) and soluble molecules (β2-Macroglobulin, Follistatin, and FLRG).
Gene ID
Accession #
P08476
Alternate Names
Source
Chinese Hamster Ovary cell line, CHO
M.Wt
Apparent molecular mass of 24 kDa in SDS-PAGE under non-reducing conditions, 14 kDa under reducing conditions, a disulfide-linked homodimer of two 116 amino acid glycosylated polypeptide chains.
AA Sequence
Gly311 - Ser426; Accession # P08476
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Formulation
Lyophilized from 0.2 μm filtered concentrated solution in 30 % acetonitrile and 0.1 % TFA.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile 4 mM HCl to a concentration of 0.1-0.5 mg/ml. Further dilutions should be made in appropriately buffered solutions.
Biological Activity
Measured by its ability to induce hemoglobin expression in K562 human chronic
myelogenous leukemia cells. The ED50 for this effect is 0.2-1.2 ng/mL.The specific activity of Recombinant Human/Murine/Rat Activin A is approximately 1 IU/μg, which is calibrated against human Activin A WHO International Standard.
Shipping Condition
Gel pack.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans.