Recombinant Human PDGF-AA

Gene ID

Accession #

Alternate Names

Source

Escherichia coli.

M.Wt

Approximately 28.8 kDa, a disulfide-linked homodimeric protein containing two 126 amino acid residues polypeptide. But it migrates with an apparent molecular mass of 33.6 kDa in SDS-PAGE.

AA Sequence

MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Formulation

Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, 300 mM NaCl, pH 6.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

Biological Activity

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. The ED₅₀ for this effect is 50-­200 ng/mL in a fluorometric assay using the redox sensitive dye.

Shipping Condition

Gel pack.

Handling

Centrifuge the vial prior to opening.

Usage

For Research Use Only! Not to be used in humans.