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Recombinant Rat Heparin-binding EGF-like Growth Factor

Recombinant Rat Heparin-binding EGF-like Growth Factor

Catalog No.

P1379

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Size	Price	Stock
10ug	$99.00	Ship with 5-10 days
100ug	$488.00	Ship with 5-10 days
500ug	$1,094.00	Ship with 5-10 days

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Background

Heparin-binding epidermal growth factor (HB-EGF)-like growth factor (EGF) is found in cerebral neurons. Its expression is increased after hypoxic or ischemic injury, which also stimulates neurogenesis. HB-EGF has been implicated as a participant in a variety of normal physiological processes such as blastocyst implantation and wound healing, and in pathological processes such as tumor growth, SMC hyperplasia and atherosclerosis. HB-EGF is an 87 amino acid mitogenic and chemotactic glycoprotein containing an EGF-like domain with six conserved cysteine residues. Rat HB-EGF shares about 76 % a.a. sequence identity with human HB-EGF.

Reference

1. Jin K, Mao XO, Sun Y, et al. 2002. J Neurosci. 22:5365-73

2. Higashiyama S, Abraham JA, Miller J, et al. 1991. Science. 251:936-9.

Information

Gene ID	25433
Accession #	Q06175
Alternate Names	HBEGF, DT-R
Source	Escherichia coli.
M.Wt	Approximately 9.7 kDa, a single non-glycosylated polypeptide chain containing 86 amino acids.
AA Sequence	DLEGTDLDLF KVAFSSKPQA LATPGKEKNG KKKRKGKGLG KKRDPCLKKY KDYCIHGECR YLKELRIPSC HCLPGYHGQR CHGLTL
Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, 300 mM NaCl, pH 7.4, 5 % trehalose.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Biological Activity	Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 × 10⁶ IU/mg.
Shipping Condition	Gel pack.
Handling	Centrifuge the vial prior to opening.
Usage	For Research Use Only! Not to be used in humans.

Quality Control

Quality Control & DataSheet

View current batch:

Purity > 95 % by SDS-PAGE and HPLC analyses.
Endotoxin: Less than 1 EU/μg of rRtHB-EGF as determined by LAL method.

Datasheet

Recombinant Rat Heparin-binding EGF-like Growth Factor

mRNA synthesis

Tyramide Signal Amplification (TSA)

Phos Binding Reagent Acrylamide

Cell Counting Kit-8 (CCK-8)

Inhibitor Cocktails

Background

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Quality Control & DataSheet

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