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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Interleukin-1 alpha (IL-1α) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1α and IL-1β binds to the same receptor and has similar but not identical biological properties. Recombinant rat IL-1 alpha contains 150 amino acids which is a single non-glycosylated polypeptide.
Reference:
1. Nicklin MJ, Weith A, Duff GW. 1994. Genomics. 19:382-4.
2. Bankers-Fulbright JL, Kalli KR, McKean DJ. 1996. Life Sci. 59:61-83.
3. Dinarello CA. 1997. Semin Oncol. 24:S9-81-S9-93.
Gene ID
24493
Accession #
P16598
Alternate Names
Source
Escherichia coli.
M.Wt
Approximately 17.8 kDa, a single non-glycosylated polypeptide chain containing 156 amino acids.
AA Sequence
SAPHSFQNNL RYKLIRIVKQ EFIMNDSLNQ NIYVDMDRIH LKAASLNDLQ LEVKFDMYAY SSGGDDSKYP VTLKVSNTQL FVSAQGEDKP VLLKEIPETP KLITGSETDL IFFWEKINSK NYFTSAAFPE LLIATKEQSQ VHLARGLPSM IDFQIS
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Formulation
Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris, 150 mM NaCl, pH 8.5.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 0.005 ng/ml, corresponding to a specific activity of > 2.0 × 108 IU/mg.
Shipping Condition
Gel pack.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans.