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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
The kit uses magnetic bead purification method, which is suitable for high-throughput and rapid recovery of RNA fragments of 100 bp ~ 50 kb in samples digested by DNase, with a recovery efficiency of up to 80%. It can effectively remove DNA fragments, primer dimers, salts, proteins and other contamination after DNenzymatic hydrolysis. The system is simple to operate and can be used with most nucleic acid purifiers, and the entire operation process can be fully automated. The transfer enzyme hydrolysis reaction solution is added to the 96-well plate, the binding solution/magnetic bead mixture is added, the nucleic acid is adsorbed to the surface of the magnetic beads, separated by the action of the magnetic field, the solution is aspirated to remove impurities, and after a quick wash, the RNA can be eluted with sterilized water or Elution Buffer, and the recovered RNA can be directly used for subsequent operations such as enzyme digestion, sequencing, hybridization, and PCR reaction.
This product can be used in the same way as the widely used Agencourt RNACLEAN XP, which can be used seamlessly to replace Agencourt RNACLEAN XP and effectively reduce the cost of your experiments.