RNase H (RNase-free)

RNase H is a ribonuclease enzyme that specifically hydrolyzes RNA phosphodiester bonds in DNA-RNA-binding chains. RNase H has a bivalent metal ion catalytic mechanism, such as Mg2+ and Mn2+ directly involved in the catalytic function. RNase H cannot hydrolyze phosphodiester bonds in single- or double-stranded DNA or RNA. This kit can be used to remove mRNA from the DNA and mRNA complex structures before the second strand of cDNA is synthesized, and to achieve pairing through complementary DNA sequences Spot-point snipping of RNA, identification of DNA-RNA hybrids, removal of poly(A) from mRNA hybridization onto poly (dT), In vitro polyadenylation reaction product studies.