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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Mycoplasmas are the smallest prokaryotic microorganism, only 0.1-0.3 μm in size. Due to their small size, mycoplasmas can penetrate rated filters (0.22~0.45 μm). Mycoplasma contamination remains a major problem in cell culture. Mycoplasmas can alter the DNA, RNA, and protein synthesis of culture cells, but they may not noticeably affect cell growth rates in many cases. Therefore, it is difficult to discover mycoplasma contamination with the naked eye. And it is a must to detect mycoplasma contamination routinely for laboratories that use cell culture frequently.
Mycoplasma Remover Reagent is a kind of natural antibiotic, which can effectively against various mycoplasmas species including Mycoplasma orale, Mycoplasma arginine, Mycoplasma hyorhinis, and Acholeplasma laidlawii. Mycoplasma Remover Reagent is very easy to use and has no toxicity to cell growth. Following treatment with Mycoplasma Remover Reagent for 2-3 weeks, mycoplasma contamination should be inhibited or eliminated.
Mycoplasma Remover Reagent is light yellow, soluble in water, and its solubility in water is approximately 15 mg/mL. The working concentration of this reagent for inhibiting mycoplasmas ranges from 0.01-0.5 μg/mL, and the working concentration for eliminating mycoplasmas ranges from 0.5-250 μg/ml. The most commonly used concentration ranges from 1-50 μg/mL. The working concentration of the reagent can be adjusted in different experiments.
Store the reagent at -20°C, stable for 3 years; store the stock solution at -20°C, stable for 6 months.