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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
The study of the components of the nucleus and cytoplasm has always been an important direction of cell research, and the components in the cell, especially after the extraction and isolation of the nucleus, can not only realize the research of intracellular protein localization, but also realize the research on the transcriptional regulation mechanism of intranuclear proteins.
This kit provides a simple and convenient method for extracting cytoplasmic and intranuclear proteins from cultured cells and fresh tissue homogenizers, which can be used for subsequent experiments such as Western blot.
Western Blot analysis of cytoplasmic protein β-tubulin and intracellular protein Lamin A of 293T cells isolated by the kit listed above.
Buffer A/C and Cell wash Buffer: Store at 4 °C; Others: Store at -20℃.