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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Sulfo-NHS-Biotin Kit (30-90 kDa) is a kit for labeling proteins such as antibodies with amino groups with biotin. Biotin molecules modified with active esters in the kit can specifically covalently label molecules such as amino-containing proteins to achieve biochemical modification of proteins and subsequent immunolabeling reactions. When this product is used to label proteins, especially immunoglobulin G (IG G), the Filtration Tube included in the kit can be used to remove inhibiting labeled reactants or low molecular weight compounds such as unreacted biotin, such as Tris, etc., to increase the labeling effect. Compared to conventional treatments such as dialysis and gel filtration, this kit results in higher recovery and higher purity of Biotin-labeled compounds.
This product is easy to operate, only ammonia-reactive biotin components need to be added to IgG and other protein antibody solutions, and incubated at 37°C for 10-15 min to achieve an average of 5-8 biotin molecular marker binding per IgG molecule, and the whole process can be completed in about 1 h. This product is only suitable for 3 0-90 kDa, 50-200 μg of protein molecular markers.
Store Sulfo-NHS-LC-Biotin (A8003) at -20°C and the other components at 4°C for 12 months.