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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
The mRNA only accounts for 1%-5% of the total RNA in eukaryotic cells, and efficient purification of mRNA is crucial for subsequent experiments. Oligo (dT) 25 Beads are specially designed for the purification of eukaryotic mRNA, which uses monodisperse superparamagnetic beads that covalently couple oligomeric dT sequences on its surface. By base pairing between the oligo DT sequence and the poly(A) tail of the mRNA, high-purity intact mRNA can be quickly separated from eukaryotic total RNA or cells, animal and plant tissues.
The dT sequence combined with mRNA can be directly used as the first chain reaction primer to synthesize cDNA, or mRNA can be eluted from the beads for various downstream molecular experiments, such as RT-PCR, RPA (ribonuclease protection assay), library construction, Northern Blot analysis, next-generation sequencing, etc.
Store at 4 °C for 12-18 months, please DO NOT freeze