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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
HyperScribe™ Co-transcription mRNA Synthesis Kit plus (ARCA, T7) is designed for quick production of ARCA capped mRNA in vitro. Capped mRNA is synthesized by co-transcriptional incorporation of Anti-Reverse Cap Analog (ARCA, Catalog No. B8175) using T7 RNA Polymerase. The capping of ARCA ensures high translation efficacy. In addition, Polyadenylation (i.e. addition of Poly(A) tail) plays an important role in the stabilization of RNA in eukaryotes and enhances the efficiency of translation initiation. This kit recommends using a DNA template with a tailed sequence at the 3' end for producting mRNA with stable poly(A) tail (typically 100-120 A).
RNA synthesized using this kit has many applications in biological experiments, such as in vitro translation, antisense RNA and RNAi experiments, RNA vaccines, RNA structure and function studies, ribozyme biochemistry, RNase protein experiments and probe-based hybridization blots.
The kit contains sufficient reagents to carry out 25 reactions, 20 μL each time. It adjusts the composition and reaction based on K1063 to achieve higher RNA yields for standard 20 μL reaction systems.
Store the components at -20°C.