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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Iron is essential for nearly all living organisms and is generally stored in the heme complex, in the centre of metalloproteins and in oxygen carrier proteins. Inorganic iron is also involved in redox reactions in the iron-sulfur clusters of many enzymes, such as hydrogenase and nitrogenase.
The Iron Colorimetric Assay Kit provides a simple, convenient and highly sensitive way for detection of Ferric ion and/or Ferrous concentration in various biological samples based on colorimetric method. In the assay, ferric carrier protein dissociates ferric into solution in the presence of acid buffer. After reduction to Fe2+, iron reacts with Ferene S to generate a stable colored (λmax = 593 nm) complex that can be easily qualified using a microtiter plate reader or a spectrophotometer at 593 nm. A specific chelate chemical in the buffer is used to block copper ion (Cu2+) interference. The assay detects iron in the linear range of 8 µM to 400 µM iron concentration in various samples.