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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Alkaline phosphatase (ALP) is a hydrolase enzyme that removes phosphate groups from nucleotides proteins and alkaloids. Changes in ALP level and activity are associated with various disease states in the bone and liver.
The Alkaline Phosphatase Activity Colorimetric Assay Kit provides a highly sensitive and convenient way for detection of ALP activity based on colorimetric method in serum and biological samples. The kit contains 10 substrate tablets providing convenience for multiple usages. The assay utilizes p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which produces yellow (λmax = 405 nm) when dephosphorylated by ALP that can be easily detected using a spectrophotometer or an ELISA plate reader. The assay can detect 10-250 µU ALP in various samples.